Mordor’s market analysis reveals that soy protein has the highest market share. Available in flour, concentrate and isolate formats, soy protein is ‘widely’ used in processed food, as a protein supplement or as protein drinks. Zander, P. et al. Grain legume decline and potential recovery in European agriculture: a review. Agron. Sustain. Dev. 36, 26 (2016). Alonge, M. et al. Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing. Genome Biol. 23, 258 (2022).

Warsame, A. O., O’Sullivan, D. M. & Tosi, P. Seed storage proteins of faba bean ( Vicia faba L): current status and prospects for genetic improvement. J. Agric. Food Chem. 66, 12617–12626 (2018).

1775

M. Shakerkhatibi, N. Mohammadi, K. Zoroufchi Benis, A. B. Sarand, E. Fatehifar and A. A. Hashemi, Environ. Health Eng. Manage. J., 2015, 2, 117–122 CAS .

In fact, it’s estimated that more than 260,000 infants born worldwide in 2015 had neural tube defects, many of which may have been preventable by adequate maternal folate intake ( 10).Z. Ma, J. Boye, B. Simpson, S. Prasher, D. Monpetit and L. Malcolmson, Food Res. Int., 2011, 44, 2534–2544 CrossRef CAS . Total RNA sequencing was carried out for three biological replicates per condition. Eighteen libraries were prepared, and paired-end Illumina HiSeq mRNA sequencing (2 × 100 bp RNA sequencing) was performed by GeneWiz, which produced around 2 × 70 million reads per library on average. Adaptor sequences were removed using CLC Genomics Workbench 11 (CLC Bio Workbench, Qiagen). Only inserts of at least 30 nt were conserved for further analysis. Reads were mapped to the Hedin/2 genome using CLC Genomics Workbench 11 according to the manufacturer’s recommendations. The mapped reads for each transcript were normalized as total counts and used for calculating gene expression. Intact and broken pairs were counted as one. The total counts of each transcript under different conditions were compared using proportion-based test statistics 86 implemented in the CLC Genomic Workbench suite. This β-binomial test compares the proportions of counts in a group of samples against those of another group of samples. Different weights were given to the samples, depending on their sizes (total counts). The weights were obtained by assuming a β-distribution on the proportions in a group, and estimating these, along with the proportion of a binomial distribution, by the method of moments. The result was a weighted t-type test statistic. We then calculated a false discovery rate correction for multiple-hypothesis tests 87. Only genes with a minimum of ten reads for all compared conditions were considered in the analysis. Orthologous gene family identification Comparison of Faba Bean Protein Ingredients Produced Using Dry Fractionation and Isoelectric Precipitation: Techno-Functional, Nutritional and Environmental Performance'​