Feng B, Lu D, Ma X, Peng Y, Sun Y, Ning G, Ma H. 2012. Regulation of the Arabidopsis anther transcriptome by DYT1 for pollen development. Plant Journal 72: 612– 624. Tissot Announces Its New Female Ambassador". Tissot Watches. October 23, 2015. Archived from the original on June 12, 2019 . Retrieved February 26, 2017. Chang Z, Chen Z, Yan W, Xie G, Lu J, Wang N, Lu Q, Yao N, Yang G, Xia J etal. 2016. An ABC transporter, OsABCG26, is required for anther cuticle and pollen exine formation and pollen–pistil interactions in rice. Plant Science 253: 21– 30.

Sina (in Chinese). July 7, 2005. Archived from the original on February 27, 2017 . Retrieved February 26, 2017.Sina (in Chinese). July 26, 2002. Archived from the original on December 22, 2011 . Retrieved July 24, 2018. Pollen development spans from microspore mother cell formation to pollen grain maturity. To identify the specific stage at which SlMPK20 exerts its role in pollen development, we performed a set of cytological experiments. With our approach, we were able to provide insights into CS functions in tumor immunity. First, we revealed the relationships between senescence levels and immune features in individual cancer types, deepening our understanding of the exact roles of CS in context-dependent mediation [ 70]. Second, CS scores showed positive correlations with total cell infiltration levels and PD-L1 expression in particular cancer types, including melanoma and prostate cancer. These prominent associations may provide possible explanations for age-dependent immune dysfunctions in these two cancer types [ 71, 72]. Third, we observed that senescent cancer cells from PRAD patients exhibited activated immune properties, showing increased expression of HLA genes and frequent communication with T-cells. These results suggest potential mechanisms underlying previous observations that immune surveillance of senescent cells enhanced immune clearance and limited tumor progression [ 70]. Further investigations based on the assessment of CS levels would facilitate mechanistic understanding at the systems level in more age-related diseases.

Odanaka S, Bennett AB, Kanayama Y. 2002. Distinct physiological roles of fructokinase isozymes revealed by gene-specific suppression of Frk1 and Frk2 expression in tomato. Plant Physiology 129: 1119– 1126. Reuscher S, Akiyama M, Yasuda T, Makino H, Aoki K, Shibata D, Shiratake K. 2014. The sugar transporter inventory of tomato: genome-wide identification and expression analysis. Plant Cell Physiology 55: 1123– 1141. Xu FQ, Li XR, Ruan YL. 2008. RNAi-mediated suppression of hexokinase gene OsHXK10 in rice leads to non-dehiscent anther and reduction of pollen germination. Plant Science 175: 674– 684. For SNVs, we used a similar multiple linear regression model and found that the CS score was correlated with lower total mutation numbers ( Supplementary Figure S2 C, available online at https://academic.oup.com/bib, coefficient=−0.18, P<2e−16). Additional investigations revealed significant correlations in 15 of 33 cancer types, including PRAD, BRCA and LUAD ( Figure 2 D). As PRAD also exhibited relatively higher CS levels ( Figure 1 C), we further selected this cancer type to analyze the differences in mutational events in PRAD patients between the low-CS and high-CS groups (divided by the median CS score). Microsatellite instability (MSI) is a molecular characteristic in hypermutated tumors due to a defect in mismatch repair genes [ 36]. Higher MSI scores were also present in the low-CS group of PRAD patients ( Supplementary Figure S2 D available online at https://academic.oup.com/bib). In general, the low-CS groups maintained more alterations per sample in PRAD ( Supplementary Figure S2 E and F available online at https://academic.oup.com/bib). Specifically, we found that missense SNVs mainly accounted for overall mutation events, especially C>T mutations. Aiming to identify the discrepancy in driver mutations between the high- and low-CS groups, we examined the top mutated oncogenic genes in individual groups, respectively ( Figure 2 E and F). TP53 and SPOP mutations were more frequent in the low-CS group of PRAD patients, which was also confirmed by experimental measurement of TP53 and SPOP mutations [ 37] ( Supplementary Figure S2 G available online at https://academic.oup.com/bib). Additionally, CTNNB1 mutations uniquely occurred in low-CS PRAD patients ( Supplementary Figure S2 H available online at https://academic.oup.com/bib). These mutations are implicated in the malignant transformation of prostate cancers [ 38], indicating the more malignant characteristics of low-CS patients. Consistently, we also found significant correlations of CS levels with DNA repair deficiency and tumor stemness indices in PRAD ( Figure 2 G). Tomato ( Solacum lycopersicum L. cv Micro-Tom) seeds were sown and grown as described previously (Chen etal., 2016). qRT-PCR analysisGelin ( 格林) (2022). "Liu Yifei: keep the characteristics" 刘亦菲：千帆过尽，依然故我. 青年文摘[ Youth Literary Digest] (in Chinese). Vol.749. Beijing: China Youth Press. pp.12–13. ISSN 1003-0565. Li Y, Zhang L, Wang X, Zhang W, Hao L, Chu X, Guo X. 2013. Cotton GhMPK6a negatively regulates osmotic tolerance and bacterial infection in transgenic Nicotiana benthamiana and plays a pivotal role in development. FEBS Journal 280: 5128– 5144.

For the bimolecular fluorescence complementation (BiFC) assay, the full-length coding sequence of SlMPK20 was PCR-amplified using the primer pair BiFC-SlMPK20-F/BiFC-SlMPK20-R (Table S1) and fused to the C-terminal or N-terminal fragment of YFP in the p2YC and p2YN vectors (Yang etal., 2007) to produce pSlMPK20-YFP C and pSlMPK20-YFP N, respectively. The same procedure was used to amplify SlMYB32 cDNA using the primer pair BiFC-SlMYB32-F/BiFC-SlMYB32-R (Table S1) and then fused to the N-terminal fragment of YFP to produce pSlMYB32-YFP N. The plasmids were transformed into A.tumefaciens strain C58C1 individually. The BiFC assay was then performed (Yang etal., 2007). Quantitative proteomic analysis by LC-MS/MS Hong Kong protesters call for 'Mulan' boycott after star went public in support of police". CNN. August 16, 2019. Archived from the original on August 18, 2019 . Retrieved August 16, 2019. Ferguson AC, Pearce S, Band LR, Yang C, Ferjentsikova I, King J, Yuan Z, Zhang DB, Wilson ZA. 2017. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytologist 213: 778. Extraction and analysis of the phytohormones indole-3-acetic acid (IAA) and jasmonic acid (JA) were performed based on a reported procedure (Durgbanshi etal., 2005; Wang etal., 2016) using an Agilent 1290 infinity HPLC system (Agilent Technologies, Waldbronn, Germany) (see Methods S6 for details). Results SlMPK20, a member of group D MAPK, is preferentially expressed in stamen Fig.S9CR-9 and WT anthers shared the same set of 1215 differently expressed genes during pre-mitotic pollen development.Mitogen-activated protein kinases (MAPKs) play important roles in modulating a variety of signal transduction pathways. The MAPK cascade consists of three consecutively kinases: MAPK, MAPK kinase (MAPKK) and MAPKK kinase (MAPKKK). MAPKs often phosphorylate transcription factors to activate relevant signaling pathways that further regulate the expression of downstream genes (Group etal., 2002; Hao etal., 2015). Phylogenetically, MAPKs can be divided into four classes. Here, groups A, B and C all have the ‘TEY’ motif and exist in both animals and plants, whereas group D is specific to plants and contains a ‘TDY’ motif (Mohanta etal., 2015). NetEase (in Chinese). May 7, 2008. Archived from the original on September 14, 2017 . Retrieved June 17, 2010. During an interview with Variety magazine at the premiere of Mulan on March 10, 2020, Liu described herself as " Asian" instead of " Chinese", which caused anger among some Chinese social media netizens, who threatened to boycott the movie and accused her of forgetting her roots. They also questioned why a non-Chinese national was playing the "legendary Chinese icon". [51] [52] Sina (in Chinese). May 22, 2017. Archived from the original on May 15, 2020 . Retrieved November 12, 2017. China Daily (in Chinese). March 25, 2013. Archived from the original on August 5, 2018 . Retrieved February 26, 2017.