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For each sample processed in Jena, a double-stranded DNA sequencing library was prepared from 20 μL of extract, with partial uracil-DNA-glycosylase (UDG) treatment (hereafter denoted as ds_halfUDG) or without UDG treatment (ds_nonUDG), following a published protocol 106. Sample-specific index combinations were added to the sequencing libraries 107, 108. The indexed libraries were shotgun sequenced on an Illumina HiSeq 4000 instrument for screening, with 75 single-end-run cycles for ds_halfUDG libraries and 75 double-end-run cycles for ds_nonUDG libraries. After screening, one ds_nonUDG library and seven ds_halfUDG libraries were deep sequenced in the University of Kiel, on an Illumina HiSeq 4000 platform with 75 double-end-run cycles using the manufacturer’s protocol. Rats vary in size depending on species. Their bodies are typically longer than 5 inches, and they rarely weigh more than 18 ounces. Smaller, thin-tailed rodents are referred to as mice. Blau M, Nagler W, Bender MA. Fluorine-18: a new isotope for bone scanning. J Nucl Med. 1962;3:332–4.Close up of a wild brown rat in autumn foraging and eating seeds in natural woodland habitat. (Image credit: Getty) Rat habits Rats can be found in a variety of habitats. Rats can be found in a variety of habitats. Some species can be found in the wilderness, such as woods and forests, as well as fields and meadows. There are also wild tree rats that live on tropical islands.

The International Union for Conservation of Nature (IUCN) lists 16 rat species on its Red List of Threatened Species. They are considered threatened with extinction due to loss of habitat and decreasing populations.

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Rats are small animals commonly found throughout Gielinor, mostly in cities, sewers, and dungeons. They have a combat level of 1. Note the eyes with the large pupil and nictitating membrane found at the inside corner of the eye. This membrane can be drawn across the eye for protection. The eyelids are like those found in humans. A common misconception about rats is that they eat everything. However, the diet of a rat depends on the species. This misconception may have developed as a result of the highly adaptable brown rat and house rat. House rats eat nearly anything digestible, especially stored grains, when living humans. Meanwhile, the brown rat is omnivorous but prefers a carnivorous diet. Brown rats hunt a wide range of prey, including shrimp, snails, mussels, insects, and bird eggs.