Introduction Since the revolutionary development of the polymerase chain reaction (PCR) in the 1980s, 1,2 nucleic acid amplification tests (NAATs) have become an indispensable tool throughout the entire life sciences field, and have even grown to be the gold standard of nucleic acid analysis, especially in clinical applications, 3–6 but also for food quality control 7 and environmental monitoring. 8 A remarkable trend, emerging between 1995 and 2005, can also be observed in the development of isothermal NAATs, which was provoked by the limitations of PCR. The complex and expensive devices required for thermal cycling and real-time detection during PCR restrict the use of this amplification method. Isothermal NAATs enable amplification reactions at constant and moderate temperatures. Simple and low-cost devices, as well as fast processing times compared to PCR, make isothermal NAATs increasingly attractive and open up new application opportunities in the field of point-of-care (POC)/point-of-need (PON) testing. 9–11 A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds usually by electrical, thermal or optical signals”. (B. Nagel, H. Dellweg and L. M. Gierasch 1992) 54,55

H. Kaneko, T. Kawana, E. Fukushima and T. Suzutani, J. Biochem. Biophys. Methods, 2007, 70, 499–501 CrossRef CAS PubMed. The analytical specificity of an NAAT describes the probability of detecting the correct target sequence rather than non-specific targets 53 Bousema T, Drakeley C. Epidemiology and infectivity of Plasmodium falciparum and Plasmodium vivax gametocytes in relation to malaria control and elimination. Clin Microbiol Rev. 2011;24:377–410.P. Sengupta, K. Khanra, A. R. Chowdhury and P. Datta, Bioelectronics and Medical Devices: From Materials to Devices - Fabrication, Applications and Reliability, 2019, ch. 4, pp. 47–95 Search PubMed. If this moment is the one that you cherish, then you can keep it close to yourself every time in your bedroom! Children love lights and when they have a light that’s as cute as a minion is, they won’t resist sitting beside it and staring! And maybe, you’d be able to do the household chores without much disturbance, then! Custom-made centrifugal microfluidic platform for the orthogonal detection of proteins and nucleic acids 82

The process of converting a biorecognition event into an electrical signal with a transducing elementTo make it interesting, the minion-style chairs and table can help! And who knows your child starts loving studying because of this little thing? You can get this table chair set HERE. Wall Decals Are A Must-Have!

C. Liang, Y. Chu, S. Cheng, H. Wu, T. Kajiyama, H. Kambara and G. Zhou, Anal. Chem., 2012, 84, 3758–3763 CrossRef CAS PubMed. Kyabayinze DJ, Zongo I, Cunningham J, Gatton M, Angutoko P, Ategeka J, et al. HRP2 and pLDH-based rapid diagnostic tests, expert microscopy, and PCR for detection of malaria infection during pregnancy and at delivery in areas of varied transmission: a prospective cohort study in Burkina Faso and Uganda. PLoS One. 2016;11:e0156954. Now that your kids (or you) are die-hard minion fans, why not cram the room with minions to the brim? Just the wallpaper magnifies the theme. Plus, it’s not that hard to re-create so even if you aren’t that creative, this is a piece of cake! Match the curtains with the bedding J. H. Jung, S. J. Oh, Y. T. Kim, S. Y. Kim, W.-J. Kim, J. Jung and T. S. Seo, Anal. Chim. Acta, 2015, 853, 541–547 CrossRef CAS PubMed.P. Prompamorn, P. Sithigorngul, S. Rukpratanporn, S. Longyant, P. Sridulyakul and P. Chaivisuthangkura, Lett. Appl. Microbiol., 2011, 52, 344–351 CrossRef CAS PubMed. Hu SF, Li M, Zhong LL, Lu SM, Liu ZX, Pu JY, et al. Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1-4. BMC Microbiol. 2015;15:265.

This is also referred to as clinical sensitivity, and describes the percentage of positive test results which are correctly identified as positive 53 H. J. Chun, S. Kim, Y. D. Han, K. R. Kim, J.-H. Kim, H. Yoon and H. C. Yoon, ACS Sens., 2018, 3, 2261–2268 CrossRef CAS PubMed. Cheng Q, Cunningham J, Gatton ML. Systematic review of sub-microscopic P. vivax infections: prevalence and determining factors. PLoS Negl Trop Dis. 2015;9:e3413. How amazing (and frightening) would it really be if minions could just break through our bedroom walls whenever we wanted to see them! Crazy, right?Cuadros J, Perez-Tanoira R, Prieto-Perez L, Martin-Martin I, Berzosa P, Gonzalez V, et al. Field evaluation of malaria microscopy, rapid malaria tests and loop-mediated isothermal amplification in a rural hospital in South Western Ethiopia. PLoS One. 2015;10:e0142842. In case, your kids’ bedroom is not too big or you need extra space every now and then, then this minion foldable chair is a smart thing to have.