A variant on one allele of this gene can cause the disease, however a variant on both alleles of this gene can result in a more severe form of the disease/phenotype. Jacco@Work • Jacob Tillberg • James Landino • James Warren • Jason Ross • Jaytech • JELO • Jerome Isma-Ae • JIKES • Joe Miller • Josh Harnois • JSTJR • Julius Dreisig • JumoDaddy Mutation carriers underwent computed tomography (CT) angiography of the aorta, main aortic branch vessels, and the arteries of the brain. One radiologist at Umeå University Hospital reviewed these evaluations. Statistical analysis The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request. Authors’ contributions

The functional myosin light chain kinase (MYLK) gene localizes with marker D3S3552 on human chromosome 3q21 in a greater than 5-Mb yeast artificial chromosome region and is not linked to olfactory receptor genes. Adams JN, Brooks M, Redpath TW, Smith FW, Dean J, Gray J, et al. Aortic distensibility and stiffness index measured by magnetic resonance imaging in patients with Marfan’s syndrome. Br Heart J. 1995;73:265–9. Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JA. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 2007;35:W71–4.Alignment of color-space reads to the human reference genome (hg19) was performed using Lifescope Genomic Analysis Software v2.1 (Life Technologies). Approximately 90 % of the reads from each sample were uniquely mapped to the target regions, generating an average coverage of 35X and a median coverage of 30X over the targeted exons across samples. Single-nucleotide variants (SNVs) and small insertions and deletions (indels) were subsequently called by the diBayes algorithm available within Lifescope. All called SNVs and indels were imported into a local installation of the CanvasDB database system for annotation and further analysis [ 8]. In a large consanguineous Arab family with thoracic aortic aneurysm and dissection, Shalata et al. (2018) identified a missense mutation in the MYLK gene (A1491S; 600922.0005) that was not found in ethnically matched controls or public variant databases. The mutation was present in homozygosity in 6 severely affected family members and in heterozygosity in 3 patients with a later age of onset; the mutation was also present in heterozygosity in 16 asymptomatic family members. The authors stated that the most appropriate designation for the mode of inheritance in this family was autosomal dominant with incomplete penetrance. Comprehensive medical history and clinical data analysis of homozygous individuals excluded congenital bladder or intestine involvement; there was thus no evidence for megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS; see below) in this family.

Sally Jay • Sam Tabor • Sara Skinner • Sarah Clark • Savage • Savoi • Saysha • Sebastian • Sebastian Lind • Seri • Shantee • Siera • Simon Latham • Sivan • SJMA Choir • SKYXXX • Slyleaf • Sofia Quinn • Sonam • Sophie Strauss • Sophiya • Spencer Ludwig • Stefan Ludik • Stella Talpo • Sundial • SYKES • Syrin • SyonA variant on the paternally-inherited allele of this gene can cause the disease, if the alternate allele is imprinted (function muted). Total RNA was extracted from the whole blood of two carriers (II:2 and II:3) and two non-carriers (II:4 and II:5) using Trizol (Life Technologies) and then converted into cDNA by using RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas, Thermo Fisher Scientific). Bidirectional Sanger sequencing (Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit, Applied Biosystems, Thermo Fisher Scientific) on a 3730xl DNA Analyzer (Applied Biosystems) was used for analysis of MYLK cDNA (NM_053025.3 ENST00000360304) using primers in exon 17 (ENSE00003507438) and 18 (ENSE00003603809) to confirm the mutation or to verify nonsense-mediated decay. Sequence analysis was performed using the Sequencher (Gene Codes Corporation, Ann Arbor, MI, USA). Primers sequences where designed using Primer 3 Plus [ 9] and are available upon request. Prediction of the effect of sequence variants on protein function was performed using MutationTaster [ 10]. Clinical examination Their is a plethora of research looking at how both added hormones, as well as the natural hormones in dairy milk influence the body. Individual II:3 suffered an aortic dissection at the age of 43 years. He was previously healthy and was suddenly affected by chest pain. CT revealed a type A aortic dissection from the ascending aorta to the proximal descending aorta. The diameter of the dissected ascending aorta was 47 mm. He received a supracoronary graft, reconstruction of the aortic arch, and an elephant trunk. There were no further cardiovascular events during 13 years of follow up. Choosing a plant-based alternative has a number of benefits over dairy milk on its own. Some people suffer from lactose intolerance or milk allergies that means they cannot consume an animal-based version- others simply do not like the taste or texture of more traditional options. Even without a direct allergy or intolerance, some people feel cow’s milk contributes to gastrointestinal issues like inflammation, Inflammatory Bowel Syndrome, Crohns, or Colitis.

Unsweetned Fortified Soy Mylk is one of two mylk substitutes that should be used for toddlers who are dependent on it (rather than 100% from food) for fat/calories/protein/vit D and Calcium. Calvertron • Cantus • Capital A • Cash Cash • Cassino & Laben • Castor Troy • Centra • Centron • Champion • Charity Strike • Chasing Dreams • Chris Luzz • CITYWLKR • Crayons • Coalesced • Cobalt Rabbit • Color Source • CPT • Cursor • Cutline

Better for the environment:

There are countless studies connecting saturated fat to CVD, so much so that the American Heart Association recommends cutting down on saturated fat laden foods for no more than 5% calories coming from saturated fat The 2-bp deletion in MYLK (c.3272_3273del) identified in the family investigated here leads to a premature stop codon and a nonsense-mediated decay of the transcript (Fig. 2). This decay should in turn result in insufficient kinase activity and possible SMC dysfunction in the aortic wall. Due to the decreased SMC contraction, the wall of the ascending aorta may not withstand the biomechanical forces from the pulsatile blood flow and may thus be at risk for rupture or dissection. Individual I:2 (male, aged 75 years) was hospitalized due to acute chest pain that migrated to the abdomen and legs. He was referred for CT imaging but experienced sudden circulatory arrest before the imaging was performed. Autopsy revealed a dissection from the sinuses of Valsalva to the abdominal aorta as well as blood in the pericardial sac. The report contained no information about the size of the aorta. Four years earlier, at the age of 71 years, transthoracic echocardiography indicated that the diameter of his ascending aorta was 39 mm. Mutation analysis confirmed that he carried the 2-bp deletion in MYLK. In a large Swedish family segregating autosomal dominant aortic dissection, Hannuksela et al. (2016) identified a 2-bp deletion in the MYLK gene causing a premature termination codon (S1091X) that was present in all 5 affected individuals as well as 9 unaffected family members. In addition, a family member with an intramural hematoma of the descending aorta did not carry the mutation.

It's so bad that 25 states have attempted to pass (with 6 succeeding) "ag-gag" laws, laws that punish whistle blowers for exposing (recording, sharing images/video) what happens behind the scenes of farms. Dairy consists of a natural sugar called lactose. As we get older, we begin to lose the enzyme that allows us to digest lactose (lactase). This is a completely natural and normal process. Did you know around 70% of the population is lactose intolerant? If so many Americans are intolerant to dairy, why does the USDA encourage every American to consume 3 cups/day, with only a footnote on non-dairy milks? Saturated FatWe here at Manna don’t want to impeach a certain diet or lifestyle upon you, but simply want to inform you to the best of our abilities and offer guidance if you’ve made the decision to make a change in your life. Of course, it is entirely up to you but I hope that the information I’ve given on Milk vs. Mylk will persuade you to make that small change, because even the smallest change can have a big impact. Heiberg E, Sjogren J, Ugander M, Carlsson M, Engblom H, Arheden H. Design and validation of Segment--freely available software for cardiovascular image analysis. BMC Med Imaging. 2010;10:1. Dairy fat has one of the highest proportions of saturated fat in the food supply -think butter, cream, whole milk, & cheese. Saturated fat has long been connected to Cardiovascular Disease (CVD), the world's biggest killer according to the World Health Organization.