Nie, Q.; Li, C.; Wang, Y.; Hu, Y.; Pu, W.; Zhang, Q.; Cai, J.; Lin, Y.; Li, G.; Wang, C.; Li, L.; Dou, Y.; Zhang, J. Pathologically Triggered in Situ Aggregation of Nanoparticles for Inflammation-Targeting Amplification and Therapeutic Potentiation. Acta Pharmaceutica Sinica B, 2023, 13(1), 390–409. doi: 10.1016/j.apsb.2022.07.013 Caruso, T., Carotenuto, M., Vasca, E. & Peluso, A. Direct experimental observation of the effect of the base pairing on the oxidation potential of guanine. J. Am. Chem. Soc. 127, 15040–15041 (2005). Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.

Seidel, C.A.M., Schulz, A. & Sauer, M.H.M. Nucleobase-specific quenching of fluorescent dyes .1. Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies. J. Phys. Chem.-Us 100, 5541–5553 (1996).Load the reaction mixture (From "Run conjugation reaction") to the top of the SepHadex G-25 column. Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. Kim, Eunha; Park, Seung Bum (2010). "Discovery of New Synthetic Dyes: Targeted Synthesis or Combinatorial Approach?". In Demchenko, Alexander P. (ed.). Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design Volume 8 of Springer Series on Fluorescence. Berlin: Springer. p.172. ISBN 9783642047022.

CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva. Holz, K., Schaudy, E., Lietard, J. & Somoza, M. M. Multi-level patterning nucleic acid photolithography. Nat. Commun. 10, 3805 (2019). Turner, D. H., Sugimoto, N. & Freier, S. M. Rna structure prediction. Annu. Rev. Biophys. Bio 17, 167–192 (1988).Perez-Gonzalez, C., Lafontaine, D.A. & Penedo, J.C. Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Front. Chem. 4 (2016).

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed While patent protection for the standard Cy series of dyes has lapsed, the trademarked Cy naming remains in place. Consequently, dyes that are identical to Cy dyes, but called different names, are now sold. Sale, lease, license or other transfer of the material or any material derived or produced from it. CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646. Ranasinghe, R. T. & Brown, T. Ultrasensitive fluorescence-based methods for nucleic acid detection: Towards amplification-free genetic analysis. Chem. Commun. 47, 3717–3735 (2011).Armitage, Bruce A. (27 January 2005). "Cyanine Dye–DNA Interactions: Intercalation, Groove Binding, and Aggregation". DNA Binders and Related Subjects. Vol.253. pp.55–76. doi: 10.1007/b100442. ISBN 978-3-540-22835-6. {{ cite book}}: |journal= ignored ( help) The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency. Lietard, J., Ameur, D. & Somoza, M. M. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA. Rsc Adv. 12, 5629–5637 (2022). Fuller, C. W. et al. The challenges of sequencing by synthesis. Nat. Biotechnol. 27, 1013–1023 (2009). CY-2 (长缨-2) is a development of CY-1, and it is based on C-802 missile, sharing the same turbo jet engine. The missile is in limited service in Chinese navy after numerous tests, the last of which was the test of the air-launched version, which was successfully completed by Harbin SH-5 in 1994. The major improvement is the range of this weapon is tripled to 30 nautical miles (56km), but the speed is reduced to subsonic level from CY-1's Mach 1.5. Just like the way CY-1 can be stored and launched from the container/launcher of C-801, CY-2 can be stored and launched from the container/launcher of C-802. The publicized data for CY-2 includes:

documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or For protein labeling, Cy3 and Cy5 dyes sometimes bear a succinimidyl group to react with amines, or a maleimide group to react with a sulfhydryl group of cysteine residues.Nizamov, T. R.; Iliasov, A. R.; Vodopyanov, S. S.; Kozhina, I. V.; Bordyuzhin, I. G.; Zhukov, D. G.; Ivanova, A. V.; Permyakova, E. S.; Mogilnikov, P. S.; Vishnevskiy, D. A.; Shchetinin, I. V.; Abakumov, M. A.; Savchenko, A. G. Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines. Pharmaceutics, 2023, 15(1), 127. doi: 10.3390/pharmaceutics15010127 any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any The additive effect of multiple Gs or Cs in a row is not immediately apparent, with GGGGG ranking #31 and #21 (0.71 and 0.64 relative intensity) and CCCCC ranking #1009 and #1021 (0.37 and 0.25 relative intensity) for Cy3 and Cy5, respectively. The top-ranking GAAAA motif identified in 5ʹ-Cy3 oligonucleotides here ranks #334 (0.58 relative fluorescence), further illustrating the significant differences between 5ʹ and 3ʹ labeling. Kaur, G., Lewis, J.S. & van Oijen, A.M. Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA. Molecules 24 (2019). Schneider, T. D. & Stephens, R. M. Sequence logos—a new way to display consensus sequences. Nucleic Acids Res. 18, 6097–6100 (1990).