Guerrero Llobet S, Bhattacharya A, Everts M, Kok K, van der Vegt B, Fehrmann RSN, et al. An mRNA expression-based signature for oncogene-induced replication-stress. Oncogene. 2022;41:1216. Balmus G, Larrieu D, Barros AC, Collins C, Abrudan M, Demir M, et al. Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome. Nat Commun. 2018;9:1700. We also verified the effect of NAT10 on the sensitivity and apoptosis of Huh-7 and Hep3B cell lines to Lenvatinib. After the treatment of siRNA and Remedelin in Huh-7 and Hep3B for 24 and 48 h, flow cytometry showed that Lenvatinib could significantly increase the apoptosis rate of Huh-7 and Hep3B (Fig. 4E–G). Zhang Y, Jing Y, Wang Y, Tang J, Zhu X, Jin WL, et al. NAT10 promotes gastric cancer metastasis via N4-acetylated COL5A1. Signal Transduct Target Ther. 2021;6:173. The total RNA of cells or tissues was extracted by the Trizol-chloroform-isopropanol method, and the RNA with D260/D280 = 1.8–2.1 met the experimental requirements. Refer to the manual of Trizol total RNA Kit (The whole gold, Beijing, China). The DNA sequence of Primers in this study are shown in Supplementary Table S2. Liquid chromatography–tandem mass spectrometry

Recent studies indicated that mRNA modification plays a key role in transcriptional regulation, stability, subcellular localization, and translation of mRNA [ 2, 3, 4, 5]. There are various chemical modifications of mRNA, including m6A [ 6], m5C [ 7], N4-Achylcytidine (ac4C) [ 8], m7G [ 9], m1A [ 10], etc. Previous studies were mostly focused on the mRNA modifications as m6A and m5C modifications, but study about the ac4C modification was still scarce [ 11, 12, 13, 14]. ac4C is a conserved chemical modification in eukaryotic prokaryotes [ 4]. Early studies suggested that ac4C mainly exists on tRNA and 18 S rRNA, but recent studies found that there is a large amount of ac4C medication on mRNA, which plays an important role in promoting protein translation, affecting RNA stability and alternative splicing, and regulating gene expression stability [ 4]. ac4C is also reported in the pathogenesis of a series of human diseases, such as osteoporosis [ 15], bladder cancer [ 16], and gastric cancer [ 17]. However, there are few studies on the role and mechanism of NAT10-mediated mRNA ac4C modification in the above studies. Makhov P, Naito S, Haifler M, Kutikov A, Boumber Y, Uzzo RG, et al. The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma. Cell Death Dis. 2018;9:374. Eble JA, Niland S. The extracellular matrix in tumor progression and metastasis. Clin Exp Metastasis. 2019;36:171. Wang L, Zhao H, Zhang L, Luo H, Chen Q, Zuo X. HSP90AA1, ADRB2, TBL1XR1 and HSPB1 are chronic obstructive pulmonary disease-related genes that facilitate squamous cell lung cancer progression. Oncol Lett. 2020;19:2115.

Features

Yang C, Wu T, Zhang J, Liu J, Zhao K, Sun W, et al. Prognostic and immunological role of mRNA ac4C regulator NAT10 in pan-cancer: new territory for cancer research? Front Oncol. 2021;11:630417. Li P, Wang J, Zou Y, Sun Z, Zhang M, Geng Z, et al. Interaction of Hsp90AA1 with phospholipids stabilizes membranes under stress conditions. Biochim Biophys Acta Biomembr. 2019;1861:457.

The TMR 24 Vdc Digital Output Module is a member of the Trusted range of Input/Output (I/O) Modules. All Trusted I/O Modules share common functionality and form. At the most general level, all I/O Modules interface to the Inter-Module Bus (IMB) which provides power and allows communication with the TMR Processor. In addition, all Modules have a field interface that is used to connect to Module specific signals in the field. All Modules are Triple Modular Redundant (TMR). Xiao X, Wang W, Li Y, Yang D, Li X, Shen C, et al. HSP90AA1-mediated autophagy promotes drug resistance in osteosarcoma. J Exp Clin Cancer Res. 2018;37:201.The Field Interface Unit (FIU) is the section of the Module that contains the specific circuits necessary to interface to the particular types of field I/O signals. Each Module has three FIUs, one per slice. For the TMR 24 Vdc Digital Output Module, the FIU contains one stage of the output switch structure, and sigma-delta (ΣΔ) output circuit for each of the 40 field outputs. Two additional ΣΔ circuits provide optional monitoring of the external field I/O supply voltage. Zhao BS, Roundtree IA, He C. Post-transcriptional gene regulation by mRNA modifications. Nat Rev Mol Cell Biol. 2017;18:31. Hepatocellular carcinoma (HCC) is the most common digestive system malignant tumor in the world, and the sixth-largest malignant tumor in China [ 1]. It is estimated 906,000 new cases and 830,000 deaths occurred worldwide in 2020 [ 1]. Radical surgery is the best treatment, but HCC is mostly diagnosed in the advanced stage, with limited treatment options and distant metastasis. Although the development of novel targeted therapies, such as lenvatinib, has improved the survival rate of HCC patients, metastasis and acquired drug resistance still lead to recurrence and poor prognosis. Therefore, it is urgent to explore the causes of HCC metastasis and drug resistance. More and more studies have shown that epigenetic regulation may play an important role in HCC. Understanding the molecular mechanisms of HCC metastasis and tumor drug resistance may provide new therapeutic targets for advanced HCC. A: The Allen Bradley T8480C is a digital AC voltage regulator that is used to control and regulate AC power to a load.