Fujisawa S., Amarasingham A., Harrison M. T., Buzsáki G. (2015). Simultaneous electrophysiological recordings of ensembles of isolated neurons in rat medial prefrontal cortex and intermediate ca1 area of the hippocampus during a working memory task. Dataset 1, 1–6. 10.6080/K01V5BWK [ CrossRef] [ Google Scholar] Drink Pyrat Rum neat. Yes, you can sip rum neat! In fact, for really good rums, this is the best way to enjoy them. A drink like Pyrat Rum has strong, sweet, orangey flavours that make it delicious on its own. You can drink it over a little ice if you wish too. Sip the rum slowly, swirl it around your mouth and enjoy its deep flavours! Nourizonoz A., Zimmermann R., Ho C. L. A., Pellat S., Ormen Y., Prévost-Solié C., et al.. (2020). Etholoop: automated closed-loop neuroethology in naturalistic environments. Nat. Methods 17, 1052–1059. 10.1038/s41592-020-0961-2 [ PubMed] [ CrossRef] [ Google Scholar]

Krizhevsky A., Sutskever I., Hinton G. E. (2012). Imagenet classification with deep convolutional neural networks. Adv. Neural Inf. Process. Syst. 25, 1097–1105. [ Google Scholar] Gulley J. M., Hoover B. R., Larson G. A., Zahniser N. R. (2003). Individual differences in cocaine-induced locomotor activity in rats: behavioral characteristics, cocaine pharmacokinetics, and the dopamine transporter. Neuropsychopharmacology 28, 2089–2101. 10.1038/sj.npp.1300279 [ PubMed] [ CrossRef] [ Google Scholar] Ilg E., Mayer N., Saikia T., Keuper M., Dosovitskiy A., Brox T. (2017). “Flownet 2.0: Evolution of optical flow estimation with deep networks,” in Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition (Honolulu, HI: IEEE; ), 2462–2470. [ Google Scholar] Muddle Orange, Cherry, Sugar & Bitters. Add Liquor, Stir, Add Ice and Top with Soda LYNCHBURG LEMONADE The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Publisher's Note

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Moura C. A., Oliveira M. C., Costa L. F., Tiago P. R., Holanda V. A., Lima R. H., et al.. (2020). Prenatal restraint stress impairs recognition memory in adult male and female offspring. Acta Neuropsychiatr. 32, 122–127. 10.1017/neu.2020.3 [ PubMed] [ CrossRef] [ Google Scholar] TD, BS, and AR designed, wrote, tested the library, and performed the analysis of the examples. RH and MG evaluated the algorithms. TD documented the library. TD, RH, MG, and AR wrote the manuscript. All authors contributed to the article and approved the submitted version. Funding Fujisawa S., Amarasingham A., Harrison M. T., Buzsáki G. (2008). Behavior-dependent short-term assembly dynamics in the medial prefrontal cortex. Nat. Neurosci. 11, 823–833. 10.1038/nn.2134 [ PMC free article] [ PubMed] [ CrossRef] [ Google Scholar] Gonzalez M. C., Rossato J. I., Radiske A., Reis M. P., Cammarota M. (2019). Recognition memory reconsolidation requires hippocampal zif268. Sci. Rep. 9, 1–11. 10.1038/s41598-019-53005-8 [ PMC free article] [ PubMed] [ CrossRef] [ Google Scholar]

Rossato J. I., Gonzalez M. C., Radiske A., Apolinário G., Conde-Ocazionez S., Bevilaqua L. R., et al.. (2019). Pkmζ inhibition disrupts reconsolidation and erases object recognition memory. J. Neurosci. 39, 1828–1841. 10.1523/JNEUROSCI.2270-18.2018 [ PMC free article] [ PubMed] [ CrossRef] [ Google Scholar] Drink Pyrat Rum with a mixer. Of course, there’s always the classic rum and coke option if you like to drink your rum in a long drink. Other sodas go well with rum too – try Pyrat Rum with ginger beer, lemonade, or tonic water. To top off your mixed drink, add a wedge of citrus fruit like lemon or lime to give a fruity edge. As Pyrat Rum XO Reserve has an especially orangey flavour, you could even garnish with an orange slice to accentuate that. or if you have own modules you would like to see in the official PyRAT distribution, please get in touch with

Funding

Levine S., Pastor P., Krizhevsky A., Ibarz J., Quillen D. (2018). Learning hand-eye coordination for robotic grasping with deep learning and large-scale data collection. Int. J. Rob. Res. 37, 421–436. 10.1177/0278364917710318 [ CrossRef] [ Google Scholar] Finally, for a cheeky take on a hot drink – try Pyrat Rum with coffee. You can mix a combination of hot coffee with sweeteners like sugar or condensed milk, spices like nutmeg, cinnamon, or cardamom, and rum like Pyrat Rum XO Reserve. Or if you’re wanting something refreshing for the summer, you could chill your coffee, and then mix any combination of these ingredients over ice instead. Insafutdinov E., Pishchulin L., Andres B., Andriluka M., Schiele B. (2016). “Deepercut: a deeper, stronger, and faster multi-person pose estimation model,” in European Conference on Computer Vision (Amsterdam: Springer; ), 34–50. [ Google Scholar] A) Image showing the trajectory of one rat for 120 s based on the snout coordinates. (B) Image showing rat body orientation during the entire object exploration session. (C) Average heatmap during the entire object exploration session. (D) Top: Object interaction across the entire object exploration session; Bottom left: Bar plot showing interaction time with objects A and A'; Bottom right: Bar plot showing the number of interactions with object A and A'. Data are expressed as mean ± SD. To plot the trajectory, the user must define a body part in the function Trajectory using the bodyPart parameter which is the column name of the chosen body part. The function Heatmap() uses the bodyPart and the parameters bins and vmax, which determine the resolution and color scale of the plot.

Since the hyperparameters are not optimized by the learning algorithm, they must be defined a priori and selected by trial and error or searching approaches. However, it must be noted that these heuristics have been proven to be useful in empirical tests ( Kobak and Berens, 2019). 3. Results 3.1. Library Features

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Sturman O., von Ziegler L., Schläppi C., Akyol F., Privitera M., Slominski D., et al.. (2020). Deep learning-based behavioral analysis reaches human accuracy and is capable of outperforming commercial solutions. Neuropsychopharmacology 45, 1942–1952. 10.1038/s41386-020-0776-y [ PMC free article] [ PubMed] [ CrossRef] [ Google Scholar]

To represent the pattern of object interaction among animal groups, the Heatmap() function can also be used to plot concatenated data, facilitating visual comparison between days, groups, or trials ( Figure 2C).To develop the PyRAT, we used datasets from the Edmond and Lily Safra International Institute of Neuroscience. Adult male Wistar rats ( n = 12) were placed in an open field arena (59x59 cm with 45 cm tall walls) for 20 min per day for 3 consecutive days. Twenty-four hours later, animals were exposed to two identical objects presented in the open field arena for 5 min. We analyzed 48 videos recorded from a top-down view perspective with a Microsoft LifeCam camera at a resolution of 640 x 480 pixels at 30 frames per second (FPS). Alongside these experiments, neural data from the dorsal hippocampus were collected. All procedures were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by a local Animal Care and Use Committee.