At the start of Despicable Me 3, the Fart Gun is used by Bob to incapacitate Mel and take the Illumination script. Items that are not available in store will take 3-5 working days (excluding weekends and bank holidays) to be delivered to your nominated store. Originally, Felonius Gru asked Dr. Nefario to develop a "Dart Gun", but Nefario misheard what Gru said and developed the Fart Gun, much to the former's chagrin. The Fart Gun is used as a threat to the Minions in Despicable Me: Minion Mayhem. Somehow, the fart gun's scent is replaced with that of bananas.

In the Jelly Lab Update, the Fart Gun's effects have been strengthened. When the player runs through its gas, the entire screen will be warped and distorted, as though covered in liquid, greatly blinding the player's view. This can be dangerous in some areas, as the player won't be able to see oncoming obstacles in his way, usually resulting in colliding into one. Also in this update, Minions will spawn and use the Fart Gun in Vector's Fortress.Nefario also commented in Despicable Me that there won't be a place for them to use it. Indeed, Fart Gun has very few applications such as for salutes and against El Macho in Despicable Me 2. Gru did not ask Nefario to develop a Dart Gun after he sees the Fart Gun, unlike when he asks Nefario to develop the Cookie Robots when Nefario accidentally made Boogie Robots. This was most likely due to the fact that the Dart Gun won't serve much use than the Fart Gun either. The 21-Fart Gun Salute is a reference to the 21-gun salute, performed with muskets at important ceremonies such as funerals and the inauguration of a new President. They're based in convenient locations including supermarkets, newsagents and train stations. Plus they're often open late and on Sundays.