Ug and Ig (Series 3), a caveman duo. Ug (Justin) always tries to invent something and often fumbles with it due to his strength, and Ig (Freddie Butterfield) has to always end up helping him. Their inventions, in doing so, are never used as how they were originally intended to be. Ug only speaks in gibberish sounds while Ig doesn't speak. Ig is a child. Milkshake Jake (Series 1), a sundae bar worker who has a messy line in milkshakes and always gets distracted by the professions of his customers. Dan Step (Series 3-present, GiggleQuiz), a tap dancer who often gets confused. He doesn't speak. The only GiggleQuiz episode he appeared in was The Lost Pirate's Treasure.

Doreen: Arthur Sleep's coworker. Her arm only appears. (Infrequent in Series 1 and 2, regular in Series 3 - present) Simon Pieman (Christmas special, Series 2), a pie deliveryman whose deliveries are done on wheels and whose pies always end up in his face.

Series 2, Episode 15

Enrico Paso Doble (Series 3-present), a dancer who isn't very good at dancing and gets distracted during his routines. He is played by Justin and doesn’t speak. (He did apologise in Series 3 Episode 1 and does say "Arrivederci!" in the Series 4 and 5 credits only.) Lady Chatalot: (Julie Alanah Brighten) King Flannel's friend. Flannel is always jealous of her and gets more than Flannel does. (Recurring in Series 1 to 2, regular in Series 3) Rapids Johnson (Gigglebox Pilot, Series 1-present, GiggleQuiz), a wildlife explorer who's always on the hunt for elusive wildlife (usually bears) but never actually gets to spot them. In the Gigglebox pilot, Rapids was called Jungle Jimbo and was Australian, parodying Steve Irwin. Rapids also appeared in the Justin's House episode, Teddy Bears' Picnic.

Ann Teak (Series 1–4, GiggleQuiz), an elderly bossy antiques expert who thinks that she knows everything about antiques. Professor Muddles (Series 1-present, GiggleQuiz), an eccentric scientist who often gets words mixed up and whose experiments never go to plan.

Series 1, Episode 15

Arthur Sleep (Showreel, Series 1-present, GiggleQuiz), a newsreader of a Northern origin who is a bad joke-teller, is always falling asleep, and is also the host of GiggleQuiz. Additionally, he appeared in the "Justin Time" showreel. Chip Monk (Gigglebox Pilot, Series 1), an eccentric pet shop worker who tries his best not to have to sell the animals in his shop to his customers. He also appeared in the original "Gigglebox" pilot. Rod and Annette (Series 2), a fisher couple. Rod (Justin) loves to catch fish but loses the catch and his wife, Annette ( Anita Dobson), loves to chat, never noticing his catch. Rod doesn't speak. Opera Oliver (Series 2), an opera-singing waiter who likes to sing about food so much that he tends to forget the condition of his hungry customers, who usually end up leaving.

Tommy Tummy: (Richard Sutton) Dina Lady's assistant who knows more about cooking than Dina Lady. (Series 1, 2, 4 and 5) Gigglebiz is a British children's comedy television programme (described as a 'live-action comedy sketch show' [5] by the BBC) consisting of five series that have been broadcast on CBeebies, [4] the BBC's younger children's channel, starting in 2009. Brown Bear: Another frequent and elusive subject of Rapids Johnson's search. (Series 3 - Present and GiggleQuiz) Dina Lady (Series 1–2, 4-present, GiggleQuiz), a homely cook who takes the names of dishes to their very literal meanings and whose assistant is Tommy Tummy. In Series 4 Dina became the host of a Great British Bake-off-esque show, with Tommy as her co-host. In Series 5 Dina became the delivery driver for a catering company.The Burrito Brothers (Gigglebox Pilot, Series 1–4), a trio of strongmen who haven't seen good days. The older two are played by Karl Magee and Gary Cross. The youngest brother, played by Justin, always unintentionally ruins their acts in some way. They don't speak, although they do make vocal sounds. They also appeared in the original "Gigglebox" pilot. (In the Gigglebox Promo, the third Burrito Brother said, "Watch the Gigglebox, on CBee-Bee-Beebies.") Giant Panda: is the frequent and elusive subject of Rapids Johnson's search. (Gigglebox Pilot Series 1 and episode 9 of Series 2) Robin Hood: (Tom Golding) Will Singalot's best friend who calls Will his enemy and hopes "That Minstrel has got to go". (Series 3 - present) The first series was broadcast in September 2009, comprising 25 13-minute episodes and 20 characters each. The first episode premiered on 7 September 2009. A Christmas special was broadcast on 15 December 2010. The second series comprised 15 episodes being broadcast in January 2011 and saw several characters disappear to make way for five new ones, replacing them. The spinoff quiz show GiggleQuiz came after Gigglebiz, premiering on 28 October 2019 and later in December 2020.

Will Singalot (Series 3-present), a minstrel and one of Robin Hood's Merry Men who infuriates Robin Hood with his nonstop singing and always spoils Robin's efforts to impress Maid Marian.King Flannel's Butler: (Simon Grover) King Flannel's long-suffering butler who tries to keep him in line - but not always successfully. (GiggleBox Pilot Series 1, 2, and 3) Postie (Series 1), a postman who always tries (but fails) to deliver the post on time. At just 3 sketches, Postie is the least frequent character in the entire series. He doesn't speak. (In Series 1, Episode 14, he did cheer and laugh.) Farmer Dung (Series 1–3), a jolly and at times, incomprehensible farmer who uses wordplay and who has an assistant called Reggie. When the showdown starts, a timer usually appears in the bottom left corner of the screen, however it didn't appear in "A Giggle Quizmas".