All Aboard"—in a speaking role credited as "Conductor" by Peter New—announces the train departing for the new station stop at Mount Aris. Etsy assumes no responsibility for the accuracy, labeling, or content of sellers' listings and products. In The Parent Map, Starlight and Sunburst take the train to Sire's Hollow, and later arrive back at the station to wait for the train after solving their friendship mission. In The Last Crusade, the Cutie Mark Crusaders take the Friendship Express to Aunt Holiday and Auntie Lofty's house. Steamer", seen engineering the Friendship Express, and the silhouette of "All Aboard" appear in The Crystalling - Part 1, Spice Up Your Life, and The Times They Are A Changeling.

In The Crystal Empire - Part 1, the Mane Six take the train to the Crystal Empire, which has reappeared after a thousand years.

In Surf and/or Turf, the Cutie Mark Crusaders and Twilight take the Friendship Express to the newly installed station stop at Mount Aris, the home of the Hippogriffs. In Flight to the Finish, "All Aboard" and "Steamer"—with a blue mane and tail and a cutie mark of a guitar—appear, and there is another unseen speaking role by Andrew Francis as "Train Conductor".

Finance is provided by PayPal Credit (a trading name of PayPal UK Ltd, Whittaker House, Whittaker Avenue, Richmond-Upon-Thames, Surrey, United Kingdom, TW9 1EH). The Friendship Express is also featured in promotional episode images and on the front and back cover of the DVD The Friendship Express and on the front cover of the DVD Friends Across Equestria. If you’ve already done that, your item hasn’t arrived, or it’s not as described, you can report that to Etsy by opening a case. In Simple Ways, Twilight accompanies Rarity to the train station to greet Trenderhoof as he disembarks the train. The Express appears in a dimensional rift opened by Midnight Sparkle as it speeds down a hill in a deserted area.Ace Point • Amira • Apple Brown Betty • Apple Bumpkin • Apple Cider • Apple Cinnamon • Apple Cobbler • Apple Dumpling • Apple Fritter • Apple Honey • Apple Leaves • Apple Munchies • Apple Split • Barber Groomsby • Beauty Brass • Berry Dreams • Berryshine • Bill Neigh • Blueberry Curls • Bumblesweet • Bushel • Business Savvy • Candy Apples • Candy Mane • Caramel • Caramel Apple • Cathy O'Mara • Charged Up • Chelsea Porcelain • Cherry Berry • Cherry Fizzy • Cherry Gold • Count Caesar • Crusoe Palm • Daisy • Dance Fever • Dane Tee Dove • Dr. Steamer" is present in the first six versions of the animated sequence for the remastered My Little Pony theme song, appearing in this sequence even earlier than his first appearance in the series proper. At the end of The Crystalling - Part 2, the same group departs from the Crystal Empire once again by way of the Express.