The animal study was reviewed and approved by Hospital Universitario A Coruña, Spain, project code P102. Author Contributions The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Publisher’s Note Commissioned officers in pay grades O-1, O-2, or O-3 who are credited with over 4 years (i.e., at least 4 years and 1 day) of prior active service as a warrant officer are entitled to the special rate of basic pay for pay grade O-1E, O-2E, or O-3E. What is Les military? In summary, we performed an in-depth analysis of the role of each individual gene of the acinetobactin metabolism in the virulence of A. baumannii ATCC 17978, allowing us to identify six potential targets for the design of new antimicrobials against this microorganism: five of them involved in its biosynthesis ( entA, basG, basC, basD, and basB) and one related to its transport ( bauA). Due to the similar function and potentially similar structure of the enzymes involved in the biosynthesis of acinetobactin and fimsbactin, inhibitors against the remaining biosynthetic steps could also have the potential to be effective by inactivating both redundant proteins. Data Availability Statement

ORCID: Kelly Conde-Pérez, orcid.org/0000-0003-1238-2221; Juan C. Vázquez-Ucha, orcid.org/0000-0003-4949-0779; Laura Álvarez-Fraga, orcid.org/0000-0003-3920-5866; Lucía Ageitos, orcid.org/0000-0002-2422-3773; Soraya Rumbo-Feal, orcid.org/0000-0002-1796-1815; Marta Martínez-Guitián, orcid.org/0000-0002-3457-0613; Noelia Trigo-Tasende, orcid.org/0000-0001-5093-8994; Jaime Rodríguez, orcid.org/0000-0001-5348-6970; Germán Bou, orcid.org/0000-0001-8837-0062; Carlos Jiménez, orcid.org/0000-0003-2628-303X; Alejandro Beceiro, orcid.org/0000-0002-6340-7815; Margarita Poza, orcid.org/0000-0001-9423-7268Kelly Conde-Pérez 1,2†‡ Juan C. Vázquez-Ucha 1†‡ Laura Álvarez-Fraga 1,3*†‡ Lucía Ageitos 4† Soraya Rumbo-Feal 1,2† Marta Martínez-Guitián 1† Noelia Trigo-Tasende 1† Jaime Rodríguez 4† Germán Bou 1† Carlos Jiménez 4† Alejandro Beceiro 1†§ Margarita Poza 1,2†§ The LES is a comprehensive statement of a member’s leave and earnings showing your entitlements, deductions, allotments (fields not used for Reserve and National Guard members), leave information, tax withholding information, and Thrift Savings Plan (TSP) information. What is Aye date? Deletion of basB and basD also led to a significant decrease in virulence characterized by an impaired fitness under iron-limiting conditions and increased mice survival. We have previously reported that basB is an essential gene for the virulence of A. baumannii during pneumonia in mice and for bacteria growth under iron-limiting conditions ( Martínez-Guitián et al., 2020). In addition, Gaddy et al. demonstrated that basD gene is essential for the biosynthesis of acinetobactin and for the bacterial growth under iron-depleted conditions in A. baumannii ATCC 19606 ( Gaddy et al., 2012). Both genes code for proteins involved in the last steps of the biosynthetic pathway of acinetobactin, where DHBA and L-Threonine precursors are linked (BasD) and the resulting intermediate is bonded to N-hydroxyhistamine (BasB) to give preacinetobactin ( Hasan et al., 2015; Shapiro and Wencewicz, 2016; Song and Kim, 2020). Both Δ basB and Δ basD mutants were unable to synthetize acinetobactin. Furthermore, a murine sepsis model was performed with the ATCC 17978 parental strain and the two double isogenic mutant strains. Mice infected with Δ basJ/Δ fbsB and Δ basF/Δ fbsC strains displayed a significantly increase of the survival rate (100% survival, P< 0.0001) in relation to those infected with the ATCC 17978 parental strain (10% survival) ( Figure 6B and Table 2). Basic Pay. Basic Pay is the fundamental component of military pay. All members receive it and typically it is the largest component of a member’s pay. A member’s grade (usually the same as rank) and years of service determines the amount of basic pay received. How does payentry help employers manage their payroll?

A murine sepsis model was used to evaluate the virulence of the A. baumannii ATCC 17978 parental strain and the isogenic mutant derivative strains as previously described ( Martínez-Guitián et al., 2019). Briefly, groups of 10 female BALB/c mice were inoculated intraperitoneally with approximately 7.5 × 10 7 colony forming units per mouse of exponentially grown cells and death was assessed during 168 h at 8-h intervals. The survival curves were plotted using the Kaplan-Meier method and analysed using the log-rank (Mantel-Cox) test. All experiments were carried out with the approval of and in accordance with the regulatory guidelines and standards established by the Animal Ethics Committee (Hospital Universitario A Coruña, Spain, project code P102). Chemical Analysis of the Siderophore Content of Acinetobacter baumannii Wild-Type and Mutant Strains Acinetobacter baumannii is one of the most common nosocomial pathogens responsible for a wide range of concerning diseases, such as pneumonia, bacteremia or secondary meningitis ( Wong et al., 2017). The rise of healthcare-associated infections caused by multidrug resistant strains of A. baumannii, together with the scarce development of new antimicrobials in the last decades, represents an important health threat ( De Oliveira et al., 2020). In fact, A. baumannii, is one of the Gram-negative ESKAPE pathogens identified by the World Health Organization (WHO) as critical priority for antibiotic discovery ( World Health Organization [WHO], 2017). When a member of the U.S. military receives an appointment as a commissioned officer, her “date of rank” is usually the date of the appointment. The appointment date is also the effective date on which the officer receives the pay and allowances for increased rank. What is considered creditable service without restrictions? Using three bowls: pour flour in the first bowl, beat two eggs in the second bowl and pour the toasted nut mix from the oven in the last bowl. Season the fish, roll it in the flour, dunk it in the egg and coat it in the nut mix. Sumac marinated stone bass with beetroot risotto recipe Our stone bass recipe – Stone bass in a nut crustde Investigaciones Científicas Avanzadas (CICA) y Departamento de Química, Facultad de Ciencias, Agrupación Estratégica CICA-INIBIC, Universidad de A Coruña, A Coruña, Spain Retired pay is calculated using your basic active service date (BASD) and pay entry basic date (PEBD),” explained the U.S. Department of Army Retirement Services. Some people assume that BASD changes, decreasing as you get closer to retirement. In reality, though, a soldier’s BASD never changes. It’s a fixed, absolute date that defines when he or she began service. So, regardless of when a soldier is planning to retire (or allowed to retire), his or her BASD will never change. It remains the same throughout the soldier’s service. Why BASD Matters

Pay Entry Base Date (PEBD) is the date that denotes how much of your service is creditable towards longevity for pay purposes. It can be found in field 4 of your last active duty Leave and Earnings Statement (LES). We thank M. I. Voskuil (Dept. of Immunology and Microbiology, University of Colorado Medical School, CO, United States) for providing pMo130. Supplementary Material If BASD is only a date, conventional wisdom may lead you to believe that it has little-to-no impact on your service. The truth, however, is that BASD affects many elements of an Army soldier’s service. First and foremost, it’s used to calculate soldiers’ active duty retirement. Generally speaking, soldiers who’ve accumulated at least 20 years of active service are eligible for retirement. To determine whether or not a soldier meets this criteria, however, you must look at the soldier’s BASD.If you’re thinking about enlisting in the U.S. Army, you may come across the term “BASD.” An acronym for “basic active service date,” it lives up to its namesake by revealing the specific date on which an Army soldier began his or her service. While many soldiers ignore this number, you should always be conscious of your BASD because it can affect other things like retirement. To learn more about BASD in the U.S. Army, keep reading. Does BASD Ever Change? de Microbiología del Complejo Hospitalario Universitario de A Coruña (CHUAC), Instituto de Investigación Biomédica de A Coruña (INIBIC), A Coruña, Spain