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Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing, 100048, China Coldewey, Devin (September 13, 2020). "Graphic video of suicide spreads from Facebook to TikTok to YouTube as platforms fail moderation test". techcrunch.com. TechCrunch . Retrieved March 24, 2022. McMillen, Lynn (September 2, 2020). "Ronald "Ronnie" Merle McNutt". Quad-Cities Daily . Retrieved April 6, 2023. Bedo, Stephanie. "Ronnie McNutt suicide video leaves kids traumatized after platforms struggle to remove it". www.news.com.au. News.com.AU . Retrieved March 25, 2022.

Dawson, Brit (September 9, 2020). "Why can't TikTok take down that disturbing suicide video?". www.dazeddigital.com. Dazed . Retrieved March 24, 2022. McCutcheon returned to music in 2017 with her fourth studio album, Lost and Found, released in August by BMG. Its lead single, "Say I'm Not Alone" was released on 30 May 2017. [30] The album peaked at number 17 in the UK, becoming McCutcheon's highest-charting record since You Me & Us. [31] Film, stage and television [ edit ] I was scrolling TikTok and suddenly there's a video of a guy that killed themselves with a shotgun and I am seriously warning you DO NOT watch it DO NOT SEARCH FOR IT because it's very terrifying and gory so pls BE CAREFUL god damn I'm shaking," another said.

Who was Ronnie McNutt?

Martine Kimberley Sherrie McCutcheon (formerly Ponting, born 14 May 1976) is an English actress and singer. She began appearing in television commercials at an early age and made her television debut in the children's television drama Bluebirds in 1989. In the early 1990s, she had minor success as one third of the pop group Milan, but it was her role as Tiffany Mitchell in the BBC's soap opera EastEnders and her role in the 2003 romantic comedy Love Actually that brought her stardom. For the former she won the National Television Award, while the latter earned her the Empire and MTV Movie awards. She was written out of EastEnders at the end of 1998 and then embarked on a pop career, this time as a solo artist. Martine McCutcheon joined EastEnders as Tiffany Raymond in 1994 (Image: BBC) Read More Related Articles Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083, China The clip has also been posted under fake titles such as "Look at the pasta I cooked tonight" or disguised as clips of animals. Han P, Zhou P, Hu S, Yang S, Yan Q, Jiang Z. A novel multifunctional α-amylase from the thermophilic fungus Malbranchea cinnamomea: biochemical characterization and three-dimensional structure. Appl Biochem Biotechnol. 2013;170:420–35.If you post a suicide video for children to find hidden in cute cat videos, there should be consequences, legal and otherwise." Described as "caring" and "loyal", Mr McNutt and his girlfriend had split up recently, Rolling Stone magazine reported. Cutinases (EC 3.1.1.74) are the smallest member of α/β hydrolase family, capable of catalyzing not only hydrolysis reactions, but also esterification and transesterification reactions [ 9]. So far, a number of cutinases have been investigated from fungi and bacteria [ 9]. In general, enzymes from thermophilic microorganisms display relatively high optimal temperature and good thermostability compared to those from mesophilic microorganisms. However, there are few reports on cutinases from thermophilic fungi, except for Humicola insolens [ 10] and Thielavia terrestris [ 11, 12]. Although many cutinase genes have been cloned and expressed, few reports are available on bioreactor systems for large-scale production [ 8, 13, 14, 15, 16]. Additionally, the production level of cutinases is still relatively low. The Thermobifida fusca cutinase expressed in Escherichia coli represented the highest yield ever reported, with an activity of 2258.5 U/mL and a protein concentration of 5.1 g/L [ 16]. To meet the needs of industrial applications and reduce production cost, high-level expression of cutinases is urgent and necessary. Pichia pastoris is an excellent protein expression host, which has been widely used for the production of various proteins, including industrial enzymes [ 17], and human interferon gamma [ 18, 19]. However, no cutinase gene has been ever highly expressed in P. pastoris suitable for commercial production. In tribute to Mr McNutt, the Celebration Church Tupelo said: “Ronnie will be missed by all who loved and knew him. Duan, X., Liu, Y., You, X. et al. High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production.