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Kupffer cells pyroptosis was detected using scanning electron microscope (SEM). The holes in the cell membrane and the rupture of the cell surface were clearly visible in the LPS group compared with the Con group, while the pyroptosis phenotypes were improved by DHA or AA supplementation (Fig. 2J). Likewise, the result of propidium iodide (PI) staining was consistent with the change in cellular phenotype (Fig. 2K). DHA/AA alleviated inflammation response in LPS-induced Kupffer cells Shore EM, Xu M, Feldman GJ, Fenstermacher DA, Cho TJ, Choi IH, et al. A recurrent mutation in the BMP type I receptor ACVR1 causes inherited and sporadic fibrodysplasia ossificans progressiva. Nat Genet. 2006;38(5):525–7. The Youden Index for any potential cut-off score is defined as the sum of the sensitivity and specificity (expressed as probabilities) of the scale at that point minus 1. The cut-off is selected as the point with the highest Youden Index. It is worth noting that this method effectively treats sensitivity and specificity as being of equal importance: false positives and false negatives are equally undesirable [ 12, 13]. Carroll BJ, Fielding JM, Blashki TG. Depression rating scales: a critical review. Arch Gen Psychiatry. 1973;28(3):361–6.

Liu, Q., Zhang, D., Hu, D., Zhou, X. & Zhou, Y. The role of mitochondria in NLRP3 inflammasome activation. Mol. Immunol. 103, 115–124 (2018). Zung’s [ 16] first mention of a cut-off score for the SDS comes in his paper entitled “How normal is depression”. Raw scores of 40 and above are considered indicative of the presence of depression. The criteria for selecting this point are not specified but Zung quotes both means and standard deviations obtained for ‘normal’ and clinical populations and also provides measures of sensitivity and specificity. The prime focus is on 20 to 64 year-olds. Here the cut-off score represents the Mean + 1.2 SD for the ‘normal’ sample and the Mean – 1.2 SD for the clinical sample. Amongst this age group, sensitivity and specificity measures for the cut-off selected are both 88%. However, Zung [ 16] also reports specificity measures of 52 and 56% for under 19 year-olds and those aged 65 and over. The current study They have also each comforted a dying female tribute in their last moments. Katniss comforted Rue, [9] Peeta comforted the female morphling, [30] and Haymitch comforted Maysilee. [28] Zhang, M. J. & Spite, M. Resolvins: anti-inflammatory and proresolving mediators derived from omega-3 polyunsaturated fatty acids. Annu. Rev. Nutr. 32, 203–227 (2012).University Children's Hospital, University Medical Centre Hamburg-Eppendorf, 20246, Hamburg, Germany.

Rutting, S. et al. Dietary omega-6 polyunsaturated fatty acid arachidonic acid increases inflammation, but inhibits ECM protein expression in COPD. Respir. Res. 19, 211 (2018).

Correction

Mo, X. L., Wei, H. K., Peng, J. & Tao, Y. X. Free fatty acid receptor GPR120 and pathogenesis of obesity and type 2 diabetes mellitus. Prog. Mol. Biol. Transl. Sci. 114, 251–276 (2013).

The 2 databases used in this study, CEMARA and PMSI, capture patient data in different ways that result in different coverage and quality. CEMARA captures diagnostic data from expert national centres (Centre of Reference “Maladies Osseuses Constitutionnelles”- CR-MOC) where FOP patients’ diagnosis is validated by physicians with expertise in the disease, through their out-patient or in-patient clinics. The PMSI system captures ICD diagnostic codes for in-patient clinics only.

Introduction

Knight RG, Waal-Manning HJ, Spears GF. Some norms and reliability data for the state-trait anxiety inventory and the Zung self-rating depression scale. Br J Clin Psychol. 1983;22(4):245–9. Plasma ALT, AST, and LDH levels were measured by using an automatic biochemical analyzer (7020, Hitachi, Japan). H&E staining

DL is employed by the company LapidusData who received a grant from Clementia Pharmaceuticals to work on the project. Consent for publication Ferrucci, L. et al. Relationship of plasma polyunsaturated fatty acids to circulating inflammatory markers. J. Clin. Endocrinol. Metab. 91, 439–446 (2006). Gong, Y. et al. Inactivation of glycogen synthase kinase-3alpha is required for mitochondria-mediated apoptotic germ cell phagocytosis in Sertoli cells. Aging (Albany NY) 10, 3104–3116 (2018).

References

Akobang AK. Understanding diagnostic tests 3: receiver operating characteristic curves. Acta Paediatr. 2007;96:644–7.