Great budget case once you replace those fans with quieter ones with rubber screws. Bear in mind that these cases are fiddly to build in due to size and will not fit a full atx motherboard. AnyDesk - A remote desktop support application that ensures secure and reliable remote desktop connections for our customer support where you may need it. Please note: You will need to generate and provide an ID for our customer support team to make a connection; this application does not allow us to connect without your consent. CiT Luna White Micro-ATX PC Gaming Case with 4 x 120mm Infinity ARGB Fans Included 1 x 4-Port Fan Hub Tempered Glass Side Panel To analyze the relative levels of 740 flavonoids and the expression levels of differentially expressed genes in the transcriptome, principal component analysis (PCA) was performed using the default parameters of the Metware data processing platform ( https://www.metware.cn/). Canonical correlation analysis (CCA) was conducted to explore the potential correlation between flavonoid metabolite levels and synthetic genes through metabolomic and transcriptomic data. Specifically, 21 flavonoid content levels and the expression levels of 41 hub genes within the flavonoid synthesis pathway of MS and MD peels at various stages were imported into Canoco 5.0 for CCA analysis, utilizing the default parameters. Quantitative real-time PCR analysis CiT Vento White Micro-ATX PC Gaming Case with 4 x 120mm ARGB Fans Included 1 x 6-Port Fan Hub Tempered Glass Side Panel

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Project and experiment design, ZW and BX. Experiment execution, QL, JY, ZL, XXY, and XYY. Data analysis, QL, YL, LL, XW, HD, and MZ. Writing, BX and QL. Review, BX and ZW. Project management, BX, GS, and ZW. All authors contributed to the article and approved the submitted version. Funding Complete with 1 x USB3.0, 2 x USB2.0, HD Audio and Card reader, all conveniently position at the top of the case, to allow easier access for the user.

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First-strand cDNA was synthesized from each RNA sample (0.2 μg). Specific primers ( Supplementary Table S1), designed by NCBI Primer-BLAST using genome sequences, were used for quantitative real-time PCR (qPCR) cycling on a CFX96 Real-Time PCR Detection System. Real-Time PCR System (Hercules, CA, USA). The qPCR cycling conditions were 95°C for 2 minutes followed by 39 cycles of 95°C for 5 seconds and 57°C for 40 seconds. Actin was utilized as an internal control ( Supplementary Table S1), and biological replicates were triplicated. Statistical analysis Citrus fruits, members of the Rutaceae family, are widely consumed throughout the world. A prime example is the sweet orange ‘Newhall’ ( C. sinensis), which stands out for its exceptional quality and has its origins in America. Citrus fruits are a rich source of bioactive flavonoids, with the peels often containing a higher concentration of these compounds than pulp and seeds ( Li etal., 2022a; Yu etal., 2022). Previous studies have shown that flavonoid compounds in Citrus peel play a significant role in anti-inflammation, anti-oxidation, immune regulation, and prevention and treatment of multiple respiratory diseases ( Peng etal., 2019; Singh etal., 2020). Unlike most other fruits, Citrus species mainly accumulate flavonone glycosides and polymethoxylated flavones (PMFs) as their main flavonoids ( Zhao etal., 2021). These compounds are highly valued as a source of common Chinese medicines, food, and nutritional supplements due to their abundance of bioactive components. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Publisher’s note RNA isolation and sequencing were performed by Metware Biotechnology Co., Ltd. (Wuhan, China). The quality of the cDNA libraries was examined, and PCR amplification and sequencing were performed using an Illumina HiSeq™ 2500 platform. The obtained reads were processed to remove contaminants and mapped to the reference genome sequence of C. sinensis v3.0 ( http://www.hzau.edu.cn) using HISAT 2.2.4. StringTie was used for transcript assembly. Fragments per kilobase per million (FPKM) values were calculated to determine gene abundance and normalize data. Differential expression analysis of mRNAs was performed using DESeq2 software. DEGs were identified based on fold change > 2 and false discovery rate (FDR) < 0.05 as cutoff values (P < 0.05). Weighted gene co-expression network analysis

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To date, efforts to identify the chemical compositions of citrus, especially flavonoid compounds, have increased significantly ( Barreca etal., 2017; Mahmoud etal., 2019; Yu etal., 2022). However, only a handful of flavonoid components present in citrus, specifically in SOPs, have been successfully identified. This limitation may hinder the growth of SOPs utilization in the food industry. Furthermore, flavonoids are a diverse group of plant metabolites that have diverse structures, wide distribution, and critical roles in plant growth, adaptation, signaling, and response to biotic and abiotic stresses ( Winkel-Shirley, 2001; Treutter, 2005; Rowan etal., 2009; Misra etal., 2010; Zhang etal., 2019). When the environment changes, flavonoid levels may also change. For example, Zanthoxylum bungeanum cv. “Fengjiao” exhibited an increase in total flavonoid content under drought stress ( Hu etal., 2021), while high solar radiation led to an increase in flavonol content in Ginkgo biloba ( Guo etal., 2020). In China, where citrus is mainly grown in subtropical and tropical regions, soil acidification and consequent magnesium (Mg) leaching are major problems ( Long etal., 2017). In addition, improper use of chemicals and inadequate use of organic fertilizers and medium and trace element fertilizers can lead to Mg deficiency in citrus, resulting in a decline in fruit quality and yield. Interestingly, Mg deficiency was found to increase total flavonoid content ( Ramakrishna and Ravishankar, 2011; Bartwal etal., 2013; Li etal., 2017; Khare etal., 2020). However, the underlying mechanism remains unexplored in SOPs. The first two principal components accounted for 50.35% (PC1) and 17.79% (PC2), respectively, and the 18 samples (including 3 replicates) were classified into 6 groups based on their developmental stage along PC1. The sample positions along PC2 were influenced by magnesium stress ( Figure3B). These findings suggest that the observed differences in flavonoid profiles were related to developmental stages and magnesium stress and were consistent with the trend in total flavonoid accumulation that peaked in MS2 or MD2 ( Figures3B, C). In addition, OPLS-DA analysis was utilized to evaluate the differences between MS and MD (Q2 = 0.99) ( Supplementary Figure2). The high Q2 value (>0.9) suggested that the OPLS-DA modules were stable and reliable and that the differences in flavonoid content could be further explored. Hierarchical clustering analysis (HCA) of the flavonoid metabolite accumulation patterns among different samples showed good repeatability within the sample group ( Figures3D). In the HCA, six clusters, corresponding to the successive stages of flavonoid metabolites in SOPs for the 18 samples, were significantly separated. The results of PCA, OPLS-DA, correlation analysis, and HCA reflected large differences between samples, high similarity among the three biological replicates, and high repeatability within samples. Differentially accumulated flavonoids metabolites in SOPs

This study was financially supported by the National Key R&D Program of China (2021YFD1600802-02), the Science and Rechnology Department of Sichuan Province, China (2021ZHCG0084), the 14th- fifth-plan of Breeding in Sichuan Province, China (2021YFYZ0023-14). Conflict of interest CiT S8-13 SFF Micro ATX Desktop Case with Mesh Front Panel 8.3 Litre 1x USB3.0 1x USB2.0 1 x 80mm Fan CiT Pro Diamond XR White Mid-Tower Gaming Case with 4mm Tempered Glass Front and Side Panels and 7 x CF120 Dual-Ring Infinity FansMass spectrometry-oriented metabolomics has emerged as a powerful tool for biological research, enabling the systematic identification and quantification of metabolites ( Alseekh etal., 2021). In this study, a widely targeted metabolomic approach combined with transcriptome data was used to explore flavonoid accumulation and its underlying molecular regulation in SOPs under magnesium stress. Although previous studies had explored the flavonoid pathway in citrus, the number of flavonoid components identified remained limited ( Yu etal., 2015; Wang etal., 2019; Yu etal., 2022). In our study, we identified 740 flavonoid components in SOPs, representing a significant supplement to the previous work. These flavonoids were classified into eight categories, with flavones (54.32%) being the most abundant class, followed by flavonols and flavanones ( Figure4A). These results indicated that they are the main flavonoid classes in SOPs and this was consistent with previous research ( Wang etal., 2019). Flavonoid carbonosides, or flavonoid glycosides, which are stable forms of flavonoids with sugar groups bound to an aglycone carbon, were also identified in high numbers (277) in SOPs. These compounds are important phytochemicals in the human diet and have been reported as active components of traditional Chinese medicines with various medicinal properties ( Shen etal., 2022), including anti-inflammatory and antioxidant activities ( Matsia etal., 2022; Wang etal., 2022). The content of Chrysoeriol-7-O-glucoside and Chrysoeriol-7-O-(6’’-feruloyl) glucoside were found to be higher in MD compared to MS during all three stages. In addition, the combined content of all flavonoid carbonosides in MD was also greater than that of MS, which correlated with the overall trend in total flavonoid content ( Figure2C). SOPs under magnesium stress showed higher contents of flavonoid carbonosides, suggesting their potential for functional food production and as a source of bioactive compounds for medication. Furthermore, SOPs also showed higher levels of flavanols, with (-)-Epicatechin-(4β->8)-(-)-epigallocatechin as the predominant component. This suggests that mandarin orange peels under magnesium stress may be a potential source for natural functional beverages and oral liquids.